This Week in Virology

Host: Vincent Racaniello Guests: Carla Giles, Zoe Dyson, Brianna McLean, and Caitlin O'Brien In Melbourne, Australia, Vincent speaks with four PhD students about their research projects and what it's like to get a doctorate down under. Video of this episode: view at YouTube This episode is sponsored by ASM Microbe and ASM Biodefense. Send your virology questions and comments to twiv@twiv.tv TWiV is a MicrobeTV Production

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler For a TWiV Thanksgiving, Vincent, Alan, and Kathy trace the feud over genome editing, a new virus discovered in human blood, and the origins of hepatitis A virus. Links for this episode Battle over genome editing (Wired) 10:05 First description of CRISPR in E. coli (J Bacteriol) 14:00 Cas genes identified (Mol Micro) 14:20 CRISPR-Cas provides immunity to bacterial viruses (Science) 15:35 Cas9-crRNA complex mediates DNA cleavage (PNAS) 16:55 Dual RNA-guided DNA endonuclease in bacterial immunity (Science) 17:50 Genome engineering using CRISPR/Cas (Science) 18:50 Cpf1 endonuclease (Cell) 23:10 Classification of CRISPR/Cas systems (Nat Rev Micro) 23:50 Summit on gene editing 21:25 Novel human virus in transfusion recipients (mBio) 27:00 Origins of hepatitis A virus in small mammals (PNAS) 55:05 Image credit Letters read on TWiV 365 7:00, 1:20:35 This episode is sponsored by ASM Microbe, ASM Biodefense, and the 32nd Clinical Virology Symposium. (1:10, 1:18:30) Weekly Science Picks 1:36:30 Kathy - Tardigrade genome sequence (video)Alan - XKCD on Gates polio eradication siteVincent - Lifting the Impenetrable Veil by Charles Calisher Listener Picks Judi - Jennifer Doudna's Ted TalkRamon - Map of the Sky Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Rich Condit, and Kathy Spindler Guests: Ralph Baric and Vineet Menachery Vincent, Rich, and Kathy speak with Ralph and Vineet about their research on the potential of SARS-like bat coronaviruses to infect human cells and cause disease in mice. Links for this episode Zoonotic potential of SARS-like bat viruses (Nature) SARS-CoV susceptibility loci via collaborative cross (PLoS Genetics) 47:00, 57:25 Ebright and Wain-Hobson quotes (Nature) 1:17:20 Misleading press release (UNC) 1:24:45 Super powerful SARS 2.0 (not!) (Motherboard) SARS-like bat virus can jump without mutating (not!) (Daily Mail) 1:26:30 Photo credit Letters read on TWiV 364 1:47:40 This episode is sponsored by Mt. Sinai Department of Microbiology (2:10), ASM Education (33:25), and the 32nd Clinical Virology Symposium (1:51:30) Timestamps by Jennifer. Thanks! Weekly Science Picks 1:53:00 Kathy - California Dept of Public HealthRich - Leafy greens on space stationVincent - Mechanical keyboards and TWiV on Perfect your podcast Listener Picks Jesse - Networking for Nerds by Alaina G. LevineRussell - Worldmapper Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiVers reveal influenza virus replication in the ferret mammary gland and spread to a nursing infant, and selection of transmissible influenza viruses in the soft palate. Links for this episode Photos of my visit to OHSU (Facebook) 6:30 Folta departs (Facebook) 9:25 T-VEC approved for melanoma (FDA) 14:45 Talimogene laherparepvec (Wikipedia) 15:10 T-VEC phase III trial (J Clin Oncol) 17:35 How drugs are named (PopSci) 16:35 Influenza transmission in mother-child dyad (PLoS Path) 23:10 Soft palate and influenza transmission (Nature) 41:00 Blausen 0872 UpperRespiratorySystem" by BruceBlaus. When using this image in external sources it can be cited as:Blausen.com staff. "Blausen gallery 2014". Wikiversity Journal of Medicine. DOI:10.15347/wjm/2014.010. ISSN 20018762. - Own work. Licensed under CC BY 3.0 via Commons." target="_blank">Image credit Letters read on TWiV 363 1:09:30 This episode is sponsored by Mt. Sinai Department of Microbiology, ASM Education, and ASM Biodefense and Emerging Diseases Conference Timestamps by Jennifer. Thanks! Weekly Science Picks 1:29:00 Kathy - Insect gearsAlan - Bye, Bye, EbolaRich - Hedy LamarrVincent - Should we resurrect extinct species? Listener Picks Jennie - Bye, Bye, EbolaPeter - Epidemics on edX Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler With their usual verve, the virus virtuosos illuminate a new method to identify all the viral nucleic acids in a sample, and regulation of viral gene expression by codon usage. Links for this episode Virome capture sequencing (mBio) 15:40 Codon usage regulates viral gene expression (PNAS) 40:00 Harold S. Ginsberg (Wikipedia) 7:15 Letters read on TWiV 362 6:50, 1:03:20 This episode is sponsored by ASM Education: ASM MOOC 5:45 Timestamps by Jennifer. Thanks! Weekly Science Picks 1:39:20 Kathy - Adam Savage, Andy Weir and Chris Hadfield talk about The MartianAlan - Project Apollo photo archive - and videoRich - Watch A Bowling Ball And Feather Falling In A VacuumDickson - California droughtVincent - Does AAAS care about Ebola anymore? Listener Picks Anonymous - Lives saved by vaccinationPaul - Science articles: A guideJudi - How a virus invades your body Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit The TWiVsters discuss Frederick Novy's return from retirement to recover a lost rat virus, and evidence for persistence of Ebolavirus in semen. Links for this episode Frederick Novy and the rat virus (Ann Int Med) 18:20 The rat virus (J Inf Dis) 22:55 Recovery of the rat virus (J Inf Dis) 35:20 Ebola sitrep 42:25 Meningitis in Glaswegian Ebolavirus nurse (ProMedMail, Medscape) 45:50 Sexual transmission of Ebolavirus (NEJM) 50:25 Ebolavirus RNA persistence in semen (NEJM) 1:04:25 Airplane banner falls into Hudson River (jpg) Image credit Letters read on TWiV 361 7:25, 1:13:25 This episode is sponsored by ASM Education: ASM MOOC 3:50 Weekly Science Picks 1:27:05 Alan - StickyRich - Arthur C. Clarke predicts the Internet (so did Vannevar Bush)Dickson - Nikon Small World Photomicrography winnersVincent - Pat Schloss on microbiome initiative Listener Picks Awkward Skeptic - 16 year old wins Google prize for detecting Ebolavirus Timestamps by Jennifer. Thanks! Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello and Kathy Spindler Guests: Michael Imperiale, Adam Lauring, and Akira Ohno Vincent visits the University of Michigan where he and Kathy speak with Michael, Adam, and Akira about polyomaviruses, virus evolution, and virus assembly, on the occasion of naming the department of Microbiology & Immunology a Milestones in Microbiology site. Links for this episode World Polio Day 3:20 Mole Day 5:25 The rat virus (J Inf Dis) 20:00 Polyomavirus miRNAs (Curr Op Virol) 39:30 Costs of GOF pause (mBio) 45:00 Lethal mutagenesis of influenza virus (J Virol) 1:03:35 Mutational robustness in RNA virus evolution (Nat Rev Micro) 52:00 Membrane binding of gag (mBio) 1:12:55 Cell proteins in retrovirus particles (J Virol) 1:23:15 Michael's twitter Adam's twitter Video of this episode - view below or at YouTube Weekly Science Picks 1:27:30 Kathy - Ada Lovelace and Charles Babbage Lego #1 and #2 and Ada Lovelace DayVincent - MiSciWriters Listener Picks Junio - XKCD marks the spotKari - Spoonflower Send your virology questions and comments to twiv@twiv.tv

Host: Vincent Racaniello Guest: Blossom Damania Vincent speaks with Blossom about her laboratory's research on Kaposi's sarcoma-associated herpesvirus, including how it transforms cells, the switch between lytic and latent replication, and its interaction with the innate immune system of the host. Links for this episode Damania Laboratory Modulation of cGAS-STING by KSHV (PNAS) KSHV vIRF1 interacts with ISG15 pathway member (J Virol) KSHV NLR homolog inhibits inflammasome (Science) KSHV inhibition of PI3K and mTOR (Blood) KSHV confers survival advantage to endothelial cells (Cancer Res) UNC Global Oncology Program Blossom on Twitter Video of this episode - view at YouTube Send your virology questions and comments to twiv@twiv.tv

Host: Vincent Racaniello Guest: Ileana Cristea Vincent meets up with Ileana at Princeton University to talk about how her laboratory integrates molecular virology, mass spectrometry-based proteomics, and bioinformatics to unravel the interplay between virus and host. Links for this episode Cristea Laboratory Ileana's work previously on TWiV 269 Blowing off steam (Cell Host Micr) Nuclear viral DNA sensors (J Biol Chem) mSystems Video of this episode - view at YouTube (coming soon!) Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Jens Kuhn Jens joins the TWiVomics to discuss the strengths and weaknesses of viral taxonomy, including its history and evolution, how viruses are ordered, and why T. rex was classified without having a living isolate. Links for this episode Jens' previous TWiV (283) 5:15 International Committee on the Taxonomy of Viruses (ICTV) 27:50 ICTV 9th Report 32:55 Classifying viruses by capsid architecture (Curr Opin Virol) 1:43:00 Image credit Letters read on TWiV 357 10:55, 1:55:40 Timestamps by Jennifer. Thanks! Weekly Science Picks 56:30, 2:07:15 Alan - The Jefferson GridRich - Summer of ScienceKathy - Bill Nye dances The ClickDickson - The MartianJens - How I Killed Pluto, Life in Our Phage World, Why the Seahorse Tail is SquareVincent - Does the public trust science? (videos, summaries) Send your virology questions and comments to twiv@twiv.tv