This Week in Virology

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Jeremy Luban The TWiV team discusses the latest data on Zika virus, including ocular defects in infants with microcephaly, and isolation of the entire viral genome from fetal brain tissue. Links for this episode Ocular findings in infants with microcephaly (JAMA and Arq Bras Oft) Evidence of Zika virus infection in brain and placenta (MMWR) Zika virus associated with microcephaly (NEJM) Hard-core sequencing (Science) Zika virus and microcephaly (virology blog) Zika virus in the Americas (NEJM) JEV and WNV transplacental infections Rubella virus facts (WHO) Share your Zika virus reagents (virology blog) No link between anencephaly and Cache Valley Virus (EID) Image credit Letters read on TWiV 376 This episode is sponsored by 32nd Clinical Virology Symposium and ASM Microbe 2016 Weekly Science Picks Alan - PhD Comics on gravitational wavesDickson - 25 years of HubbleVincent - Amazon AWS TOS and Confirming EinsteinRich - Mosquito mass productionKathy - TileAppJeremy - IL17A and autism and The Perfect Theory by Pedro Ferreira Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Guests: Carolyn Coyne, Helen Lazear, and Jeremy Luban The TWiVziks present everything you want to know about Zika virus, including association of infection with microcephaly and Guillain-Barré syndrome, transmission, epidemiology, and much more. Links for this episode Zika outbreak in Brazil (EID) 13:40 Zika summary (ProMedMail) 19:30 Zika and microcephaly (MMWR) 27:30 Facts about microcephaly (CDC) Brazil Ministry of Health microcephaly numbers Zika spread in Americas (MMWR) Deu Zica 42:30 Microcephaly in Brazil (Lancet) 46:10 GM mosquitoes did not start Zika outbreak (Discover) 1:31:30 Debunking myths around Zika (Mad Virologist) Sexual transmission vs sexually transmissible (virology blog) 1:22:10 CDC Zika travel guidance 1:15:45 CDC on Zika and sex 1:17:10 Image credit: Esper Kallas, University of Sao Paulo Letters read on TWiV 375 This episode is sponsored by 32nd Clinical Virology Symposium and ASM Grant Writing Webinar Weekly Science Picks 1:50:45 Alan - Jetstream Online School for WeatherVincent - New virus watercolors from Michele Banks (one, two)Rich - The Mad Virologist (Facebook, blog)Kathy - Orcas in AntarcticaJeremy - Pardis Sabeti on TEDHelen - Science ValentinesCarolyn - Three letter word missing from Zika warnings Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVniks consider the role of a cell enzyme that removes a protein linked to the 5'-end of the picornavirus genome, and the connection between malaria, Epstein-Barr virus, and endemic Burkitt's lymphoma. Links for this episode Divergent requirements for removing VPg (mBio) Bond, covalent bond (TWiV 210) Link between malaria and endemic Burkitt's lymphoma (PLoS Path) Multifactorial role of malaria in Burkitt's lymphoma (PLoS Path) Plasmodium infection promotes AID-dependent B cell lymphoma (Cell) Children's cancer dependent on climatic factors (Nature) Denis Burkitt (Wikipedia) Request for PACE trial data (virology blog) Letters read on TWiV 374 This episode is sponsored by 32nd Clinical Virology Symposium and ASM Grant Writing Webinar Weekly Science Picks Alan - Indoor skydivingVincent - Cancer Virus by Crawford, Johannessen, and RickinsonRich - WitKathy - The Only Woman in the Room by Eileen PollackDickson - Show everyone your clinical data Send your virology questions and comments to twiv@microbe.tv

Host: Vincent Racaniello Guest: Julius S. Youngner Vincent speaks with Julius about his long career in virology, including his crucial work as part of the team at the University of Pittsburgh that developed the Salk inactivated poliovirus vaccine. Links for this episode Trypsin for cell cultures (Proc Soc Exp Biol Med) Interferon induction by nonviral stimuli (J Gen Physiol) Interferons of different molecular weight (Proc Soc Exp Biol Med) Poliovirus thermal mutants (J Bacteriol) Combination of different cells in culture (Science) Equine influenza vaccine (Eq Vet J) Younger role in Salk vaccine (interview, amednews) Cutter Incident (JRSM) Video of this episode - coming as soon as I can get home! Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The TWiV-osphere introduces influenza D virus, virus-like particles encoded in the wasp genome which protect its eggs from caterpillar immunity, and a cytomegalovirus protein counters a host restriction protein that prevents establishment of latency. Links for this episode Ebolavirus update (ProMedMail) Influenza D virus (mBio) Vincent's influenza C virus paper (J Virol) Virus-like particles protect wasp eggs (Sci Adv) CMV protein foils cell defense against latency (Sci Adv) Image credit: Mark Letters read on TWiV 372 This episode is sponsored by ASM Grant Writing Webinar and ASM Biodefense Weekly Science Picks Alan - Go viralVincent - 3D virus popsicles (3ders, dangerous popsicles)Rich - Galileo thermometerKathy - Thing Explainer by Randall Munroe Listener Picks Christiane - Using PowerPoint to shine Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVologists discuss the finding of a second transmissible cancer in Tasmanian devils, and development of new poliovirus strains for the production of inactivated vaccine in the post-eradication era. Links for this episode Second transmissible cancer in Tasmanian Devils (PNAS) New strains for production of IPV (PLoS Path) Synchronized switch to bivalent OPV (polioeradication.org) Virologists, start your poliovirus destruction (virology blog) Image is a painting by George Kush Letters read on TWiV 371 This episode is sponsored by ASM Grant Writing Webinar and ASM Biodefense Weekly Science Picks Alan - Skyfaring by Mark VanhoenackerVincent - Your Cells. Their Research. Your permission?Rich - NASA UV photos of the sunKathy - Aurora bike lightsDickson - Unfilled vials Listener Picks Kevin - John Oliver on nutritional supplementsJim - Tour of Australian synchrotron Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler The TWiVomics review ten captivating virology stories from 2015. Ten virology stories from 2015 Recoding to make vaccines and study viral functions: TWiV 351: The dengue codeTWiV 362: Gotta catch ’em all and new paper Defining the virome: TWiV 365: Blood, feuds, and a foodborne disease, TWiV 362: Gotta catch ’em all, TWiV 356: Got viruses?, TWiV 355: Baby’s first virome, TWiV 342: Public epitope #1, TWiV 323: A skid loader full of viromes Ebola outbreak wanes, vaccines tested, persistence noted: TWiV 361: Zombie viruses on the loose, TWiV 349: One ring to vaccinate them all, TWiV 341: Ebolavirus experiences, TWiV 336: Brought to you by the letters H, N, P, and Eye, TWiV 335: Ebola lite Spillovers and scares: TWiV 347: Rose rosette and squirrel roulette, TWiV 351: The dengue code, TWiV 364: It’s not SARS 2.0, TWiV 365: Blood, feuds, and a foodborne disease The “Gain of Function” debate continues: TWiV 321: aTrip and a pause, TWiV 351: The dengue code, TWiV 354: The cat in the HAART, TWiV 363: Eat flu and dyad, and the NSABB’s new draft report Detailed mechanisms of pathogenesis: TWiV 336: Brought to you by the letters H, N, P, and eye, and TWiV 345: How a vaccine got the nod - plus numerous episodes (324, 328, 329, 330, 339, 343, 349, 353, 369) where we talked about basic research that undergirds these clinically important discoveries. Dengue vaccines: Trial TWiV 319: Breaking breakbone. Dengvaxia was licensed in December in Mexico, Philippines, and Brazil (12/28); paper we didn’t have time to cover about broadly neutralizing Abs from viremic patients and another paper from same group with X-ray crystal structure of Ab + gpE. Why do some RNA viruses undergo antigenic variation and others do not? TWiV 340: No shift, measles TWiV 346: A double helical career TWiV 345: How a vaccine got the nod Letters read on TWiV 370 This episode is sponsored by ASM Microbe and ASM Biodefense Weekly Science Picks Alan - Scientifics onlineVincent - Giant squid in Tokyo HarborKathy - A Year of Sky Listener Picks Daniel - Paper LifeStephen - How to deal with science denialistsVenkat - Kabisa Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler A swarm of virologists discusses testing of a MERS coronavirus vaccine for camels, and how a neuronal stress pathway reactivates herpes simplex virus. Links for this episode MERS coronavirus vaccine for camels (Science) WHO MERS summary Neuronal stress pathway and herpes simplex virus reactivation (Cell Host Micr) Stress flips a chromatin switch (Cell Host Micr) How herpes simplex virus reactivates (UNC press release) Histone antibodies database New antibody portal (UNC press release) Image credit Letters read on TWiV 369 This episode is sponsored by Clinical Virology Symposium Weekly Science Picks Alan - SpaceX Falcon 9 landingVincent - Scientists: Engage the public! and Lego contest winnersKathy - Google Ngram viewer Listener Picks Dennis - Americapox: The missing plagueMark - WeatherSpark and Quasispecies theory Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler A plaque of virologists explores the biology of Zika virus and recent outbreaks, and the contribution of a filamentous bacteriophage to the development of biofilms. Links for this episode More cowbell (Wikipedia) Microcephaly in Brazil (Outbreak News) Non-vector borne Zika transmission (EID) Zika virus outside Africa (EID) Zika virus possible sexual transmission (EID) Zika virus in saliva (J Clin Virol) Dengue's cousin Zika (Micr Inf) Filamentous phage promote biofilm assembly (Cell Host Micr) Biofilm history (MSU) Pseudomonas phage Pf1 Image credit Letters read on TWiV 368 This episode is sponsored by Clinical Virology Symposium and ASM Microbe Weekly Science Picks Alan - Twelve days of norovirusVincent - This Week in EvolutionRich - In the Kingdom of Ice by Hampton SidesKathy - Kajanjian lecture on Frederick Novy and Milestones ceremony at UM Listener Picks Peter - What if anti-vax, anti-science movement won?Stephen - Scandalous origin of vaccinesPritesh - Absurd way of doing business Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guests: Carolyn Coyne and Coyne Drummond Two Coynes join the TWiV overlords to explain their three-dimensional culture model of polarized intestinal cells for studying enterovirus infection. Links for this episode Carolyn and placentas on TWiV 193 Three-dimensional cell culture model (mSphere) Bioreactor video (JoVE) Neurovirologist Richard T. Johnson, 84 (Hopkins and virology blog) Tardigrade HGT disputed (BioRxiv) Exaptation of archaeal virus capsid protein from Cas4 (Biol Dir) Image credit Letters read on TWiV 367 This episode is sponsored by ASM Microbe Weekly Science Picks Kathy - Tardigrade genome sequence (video)Alan - Antibiotic action nonprofit groupVincent - Ex Machina and genome editing moratoriumRich - Launch photography by Ben CooperKathy - HIV life cycle in video (paper)Dickson - 2015 Nobel Lectures Physiology or MedicineCarolyn - Metapneumovirus entry Listener Picks Tom - Global host-pathogen databaseTrudy - Madame Curie by Eve Curie Send your virology questions and comments to twiv@twiv.tv