This Week in Virology

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler The TWiXers discuss a study on vertical transmission of Zika virus by Aedes mosquitoes, and uncovering Earth's virome by mining existing metagenomic sequence data. Become a patron of TWiV! Links for this episode Roger Tsien, 64 (UC San Diego Health) CDC running out of Zika money (NYTimes) Zika virus in Miami mosquitoes (NYTimes) No Zika virus in Rio Olympics (NYTimes) Vertical transmission of Zika virus in Aedes aegypti (AJTMH) Uncovering Earth's virome (Nature) Image credit (Global water volume) Letters read on TWiV 405 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completel free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. 0:25, 58:55 This episode is also brought to you by Drobo, a family of safe, expandable, yet simple to use storage arrays. Drobos are designed to protect your important data forever. Visit www.drobo.com to learn more. Listeners can save $100 on a Drobo system at drobostore.com by using the discount code Microbe100. Weekly Science Picks Alan - The VizziesDickson - NASA Spinoff Kathy - Maps.meVincent - Zika: The Emerging Epidemic by Donald McNeil Listener Picks Tarwin - Darwin's Radio by Greg Bear (paper or Kindle)Bodham - The start of scientific publishing, 1665 Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit From the twiVivants, follow up on FluMist and Zoster vaccines, Zika virus update, and isolation of a multicomponent animal virus from mosquitoes. Become a patron of TWiV! Links for this episode FluMist problem (FDA) DA Henderson obituary (NY Times) DA Henderson, smallpox eradicator (TWiV Special) Efficacy of VZV vaccine (Ther Adv Vaccines) Zika virus burden, Puerto Rico (JAMA) Zika virus in Miami Beach (STAT) Congenital brain anomalies and Zika virus (Radiology) Congenital Zika syndrome with arthrogryposis (BMJ) Multicomponent animal virus from mosquitoes (Cell Host Microbe) Breaking rules of infection (NPR) Image credit Letters read on TWiV 404 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completel free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. This episode is also brought to you by Drobo, a family of safe, expandable, yet simple to use storage arrays. Drobos are designed to protect your important data forever. Visit www.drobo.com to learn more. Listeners can save $100 on a Drobo system at drobostore.com by using the discount code Microbe100. Weekly Science Picks Alan - Sally Le PageDickson - Comfortably Numb by Jamie Dupuis Rich - Learned HelplessnessVincent - Virus by Marilyn Roossinck Listener Picks OneTime - The Stranglers - Old CodgerSteve - A Poem About Pronunciation Send your virology questions and comments to twiv@microbe.tv

Donald "D.A." Henderson, a physician, educator, and epidemiologist who led the World Health Organization's campaign to eradicate smallpox, died at 87 years of age on Aug. 19, 2016. Vincent was fortunate to have the opportunity to speak with DA Henderson in 2014 about his career, the smallpox eradication effort, and what it means for the eradication of polio. Become a patron of TWiV! This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completel free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. This episode is also brought to you by Drobo, a family of safe, expandable, yet simple to use storage arrays. Drobos are designed to protect your important data forever. Visit www.drobo.com to learn more. Listeners can save $100 on a Drobo system at drobostore.com by using the discount code Microbe100. Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Alan Dove, and Rich Condit The TWiV team takes on an experimental plant-based poliovirus vaccine, contradictory findings on the efficacy of Flumist, waning protection conferred by Zostavax, a new adjuvanted subunit zoster vaccine. Become a patron of TWiV! Links for this episode Tribunal orders release of PACE trial data (Valerie Eliot Smith) Our request for PACE trial data (virology blog) GM mosquito release on ballot (NPR) Florida Keys Mosquito Control District on TWiV #111 Cold chain and virus free plant based polio vaccine (Plant Biotechnol J) Disappointing flu vaccine effectiveness (CBC) ACIP votes down use of LAIV (CDC) LAIV as effective as inactivated flu vaccine (Ann Int Med) Flumist does work (NPR) Declining effectiveness of zoster vaccine (J Inf Dis) Efficacy of adjuvanted subunit zoster vaccine (NEJM) Risk of zoster from vaccine (J Inf Dis) Image credit: ViralZone Letters read on TWiV 403 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completel free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. This episode is also brought to you by Drobo, a family of safe, expandable, yet simple to use storage arrays. Drobos are designed to protect your important data forever. Visit www.drobo.com to learn more. Listeners can save $100 on a Drobo system at drobostore.com by using the discount code Microbe100. Weekly Science Picks Alan - To Scale: The Solar SystemRich - Route 66 Goes SolarVincent - Race for a Zika Vaccine by Siddhartha Mukherjee Listener Picks Bohdan - Penn and Teller on VaccinationsBill - Connections, Episode 1 Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit Polio returns to Nigeria, Zika virus spreads in Miami, and virus infection of plants attracts bumblebees for pollination, from the virus gentlepeople at TWiV. Become a patron of TWiV! Links for this episode Marian Horzinek, 80 (ProMedMail) Polio in Nigeria (Atlantic, Stat, ProMedMail) Zika virus spreads in Miami (NYTimes) More Zika virus vaccines (Science) Back to work on Zika, Congress ! (Asbury Park Press) Lack of funding will stymie Zika vaccines (Wash Examiner) FDA OK on genetically modified mosquitoes (FDA) CMV attracts aphid vectors (TWiV #70) CMV attracts pollinating bumblebees (PLoS Path) Earth dwarfed by solar flare (jpg) Letters read on TWiV 402 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completel free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. This episode is also brought to you by Drobo, a family of safe, expandable, yet simple to use storage arrays. Drobos are designed to protect your important data forever. Visit www.drobo.com to learn more. Listeners can save $100 on a Drobo system at drobostore.com by using the discount code Microbe100. Weekly Science Picks Alan - Solar storm that launched US space weather scienceRich - Data storage on DNA Dickson - Perseid meteor show liveVincent - In the Company of Microbes by Elio Schaechter Listener Picks Neva - I Contain Multitudes by Ed YongAmanda - American Gut Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Zika virus spreads in the USA, a Zika virus DNA vaccine goes into phase I trials, and how mosquito bites enhance virus replication and disease, from the friendly TWiFolk Vincent, Dickson, Alan, and Kathy. Become a patron of TWiV! Links for this episode Beverly Griffin, 86 (1 2 3) Zika virus in the US (CDC) Zika virus spreading in Miami (CDC) Zika virus in Florida (NY Times) NIH begins phase I trial of Zika virus DNA vaccine (NIAID) Mosquito salive enhances West Nile replication in mice (J Virol) Mosquito bites enhance virus infection (Immunity) Photo of A. aegyptii and mouse ears from Marieke Pingen Letters read on TWiV 401 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completel free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. This episode is also brought to you by Drobo, a family of safe, expandable, yet simple to use storage arrays. Drobos are designed to protect your important data forever. Visit www.drobo.com to learn more. Listeners can save $100 on a Drobo system at drobostore.com by using the discount code Microbe100. Weekly Science Picks Alan - The DoubleclicksKathy - WHO ZIKV app and ZIKV Giant Microbe Dickson - Photographer Rosamond PurcellVincent - Spillover: Zika, Ebola, and Beyond Listener Picks Ricardo - Portugal Four Days on Renewable EnergyFatma - DeNovo: Predicting virus-host protein interactionsStephen - How mosquitoes use six needles to suck your blood Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Harold Varmus The TWiV team is together in New York City for a conversation with Nobel Laureate Harold Varmus about his remarkable career in science. Become a patron of TWiV! Links for this episode Varmus Laboratory Varmus and Kandel on Charlie Rose (transcript) Rescuing biomedical research Letters read on TWiV 400 Video of this episode at YouTube This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. This episode was made possible by PLoS Pathogens - the leading Open Access journal to publish breakthroughs in understanding pathogens and their interactions with host organisms and each other. PLOS Pathogens fosters the open exchange of ideas across fields, publishing original research on viruses, bacteria, fungi, parasites, and prions. For more information, check out www.plospathogens.org Weekly Science Picks Alan - A year on EarthRich - TWiV #1: West Nile Virus Dickson - Chasing storms, chasing beautyKathy - Bioartography and FASEB image and video competitionVincent - The Art and Politics of Science by Harold Varmus (free download) Listener Picks Trudy - Scientific Studies on Last Week Tonight with John Oliver Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit The latest Zika virus news from the ConTWiVstadors, including a case of female to male transmission, risk of infection at the 2016 summer Olympics, a DNA vaccine, antibody-dependent enhancement by dengue antibodies, and sites of replication in the placenta. Links for this episode Zika virus disease in the US (CDC) Female to male Zika virus transmission (CDC) Zika virus in female genital tract (Lancet) Zika virus transmission in Utah (CDC and NYTimes) Olympic Zika virus risk (CDC) 15 companies on Zika virus vaccines (WSJ) Zika virus DNA vaccine in mice (Nature) Zika virus disease in Colombia (NEJM) Dengue virus antibody dependent enhancement of Zika virus (PNAS) Zika virus antibodies enhance dengue virus disease (Science) Zika virus in human placenta and developing brain (bioRxiv) Zika virus targets different human placenta cells (Cell Host Microbe) Letters read on TWiV 399 This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Become a patron of TWiV. Weekly Science Picks Alan - CDC postmortem on Ebolavirus outbreakRich - Refutations to anti-vaccine memes (Twitter, Facebook) Dickson - History of urbanizationVincent - How to cut subject from background in Photoshop Listener Picks Marion - Skeptics Guide to the Universe podcastJennie - Leatherback turtles in Costa Rica Send your virology questions and comments to twiv@microbe.tv

Host: Vincent Racaniello Guest: Sandy Weller Vincent speaks with Sandy Weller about her career and her work on the mechanisms of synthesis, maturation and cleavage and packaging of viral DNA genomes. Become a patron of TWiV! Links for this episode Cell killing by avian leukosis virus (Sandy's first paper, J Virol) ND10 protein and herpes simplex virus replication (J Virol) Recombination promoted by DNA viruses (Ann Rev Micro) HSV-1 and DNA damage response (Fut Virol) Video of this episode at YouTube This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Send your virology questions and comments to twiv@microbe.tv

Host: Vincent Racaniello Guest: David Tuller Vincent speaks with David Tuller about flaws in the U.K's $8 million PACE trial for chronic fatigue syndrome, and efforts to have the trial data released. Become a patron of TWiV! Links for this episode Science and journalism (TWiV 111) Chronic fatigue syndrome and the CDC (virology blog) PACE trial (Lancet) Trial by error part one, two, three (virology blog) PACE economics analysis (PLoS One) All articles by David Tuller at virology blog Valerie Eliot Smith's blog Karina Hansen, prisioner of Denmark This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE. Send your virology questions and comments to twiv@microbe.tv