This Week in Virology

Brianne and Vincent tackle two studies that utilize infectious viruses to examine zoonotic potential of Bombali virus, a new ebolavirus from an insectivorous species in Sierra Leone, and a human mumps-like virus from an African flying fox in DRC. Hosts: Vincent Racaniello and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Pan American Society for Clinical Virology San Diego and Houston Regional Meetings It's not SARS 2.0 (TWiV 364) Bats out of hell (TWiV 183) Bombali virus, a new Ebolavirus (Nat Micro) Asymptomatic Ebolavirus infections (virology blog) Bat-borne mumps virus (Cell Rep) Image credit Letters read on TWiV 517 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Ed Yong on AFM Vincent - Goggles Optional podcast (especially #243) Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Lonya and Jeremy take the TWiV team beTWIXt primate immunodeficiency virus proteins Vpx and Vpr and how they counteract transcriptional repression of proviruses by the HUSH complex. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, Kathy Spindler, and Brianne Barker Guests: Leonid Yorkovetskiy and Jeremy Luban Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Pan American Society for Clinical Virology San Diego and Houston Regional Meetings Vpr and Vpx counter HUSH (Nat Micro) Vpr and Vpx counter HUSH (bioRxiv version) Schematic of HUSH complex (Lehner lab) Olive colobus monkey sound credit Image credit: William (Ted) Diehl Letters read on TWiV 516 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - The Chimp and the River by David Quammen Rich- James Cook's First Voyage Journal (20 sec Google Earth summary) Kathy- High resolution Antarctica map NYTimes 46MB Maps (more info and 43 tb maps) Dickson- Jellyfish Jeremy - Pictorial Guide to Living Primates; All The World's PrimatesLonya- Chougui et al. HIV-2/SIV Vpx counters HUSH Vincent - Research!America Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The TWiV team notes the passing of Tom Steitz, an outbreak of acute flaccid myelitis in the US, a continuing Ebola virus outbreak in DRC, respiratory vaccinia due to inhalation of ground up rabbit skin, and how a human papillomavirus capsid protein directs virus-containing endosomes towards the nucleus. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Sea Phages program and application materials Tom Steitz, 78 (NY Times) AFM outbreak, US (CIDRAP) AFM surveillance (CDC) Acute Flaccid Paralysis and Enteroviral Infections (Curr Inf Dis Rep) EV-D68 neurotropism (bioRxiv) Ebola virus outbreakcontinues in DRC (CIDRAP) Vaccinia virus respiratory infection (Int J Inf Dis) HPV L2 penetrates endosome, brings virus retro (Cell) Letters read on TWiV 515 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Germ City-Microbes and the Metropolis Museum Exhibition Alan- Nikon 2018 Small World in Motion competition Kathy- Ion Torrent Sequencing (video) Dickson- 2018 Nobel Prizes Economics Kathy- Ocean-centric map Vincent - Intelligence Matters Podcast: Anthony Fauci on threats from epidemics Listener Picks Gonsalo - Cells at Work (Manga, Anime) Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The TWiVumvirate reviews this years crop of Nobel Prizes, and how cells prevent leakage of mitochondrial double-stranded RNA into the cytoplasm, which would otherwise lead to the production of interferon. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Sea Phages program and application materials Plant biologists penalized by CNRS (The Scientist) 2018 Nobel Prize in Medicine (pdf) 2018 Nobel Prize in Chemistry (pdf) 2018 Nobel Prize in Physics (pdf) 2018 Nobel Peace Prize (Nobel) Mitochondrial dsRNA triggers IFN (Nature) Letters read on TWiV 514 Timestamps by Jolene. Thanks! Weekly Science Picks 1:28:33 Alan- Compound Interest chemistry graphics Kathy- Writing letters of recommendation Guidelines Trix & Psenka Schmader et al. Madera et al. Dickson- Japan Fireworks Vincent - The Game of T-Cells and Apple Park Lego Listener Picks Steve - Shomu's Biology Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

From the 13th International Symposium on dsRNA viruses in Belgium, Vincent speaks with Harry Greenberg about his career and his work on rotaviruses, noroviruses, hepatitis B virus, and influenza virus. Host: Vincent Racaniello Guest: Harry Greenberg Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode 13th dsRNA Virus Symposium Hepatitisin blood donors (Lancet) Interferon treatmentof hepatitis B (NEJM) Mycoplasmamutantsin volunteers (PNAS) Norwalk virus infectionof chimpanzees (J Med Virol) Rotavirus rescueby reassortment (PNAS) Cold-adaptedinfluenza vaccine (Virol) Rotavirus correlates of protection(Curr Opin Virol) Rotavirus vaccine and intussusception(NEJM) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Anne Simon joins the TWiV team to talk about plant viruses, including plum pox virus that devastates nut and stone fruit trees, and a geminivirus protein that regulates viral DNA synthesis. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, Brianne Barker, and Anne Simon Click arrow to play Download TWiV 512 (62 MB .mp3, 103 min) Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Support Viruses Gordon Conference3:38 Potyvirus (ViralZone) Plum pox (Wikipedia) History, biology, management of plum pox (Mich St Ext) Papaya ring spot virus (Bus Man) Geminivirus (ViralZone) Geminivirus rep impairs PCNA SUMOylation (J Virol) Image credit Letters read on TWiV 512 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Why are so many genes unstudied? (Zimmerand Yong) Alan - Unveiling the RNA World Rich - Spaghetti mystery solved (PNAS paper) Dickson - Science Fair Vincent - A PhD Lab Coat Ceremony Listener Picks Stephen - Flu vaccine myth rant by Crislip Jolene - Research Matters in PLoS Path and PLoS Biology Nicholas - Charity Matters app Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Ned Landau joins the TWiV team to discuss restriction of HIV replication by SAMHD1, and a viral antagonist that can be used to produce a dendritic cell vaccine. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Brianne Barker Guest: Ned Landau Become a patron of TWiV! Links for this episode Support Viruses Gordon Conference TWiV #124 September 2008 SAMHD1 restricts HIV-1 replication (Nature) SAMHD1 restricts diverse retroviruses (Retrovirology) Vpx degradation of SAMHD1 independent of uncoating (J Virol) Transduction of myeloid cells with Vpx vector (Gene Ther) Letters read on TWiV 511 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne- Online Immunology Course Alan- Bacteria Builder Rich- 2018 Ig Nobel Prizes Dickson- Between Fear and Hope Ned - HUSH silencing of transposons Vincent - Paywall publishing ban (Schlitz statement); Curry on DORA Listener Pick Steve- Paywall: Business of Scholarship and Biohazard Islam- Peer review Twitter post Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Vincent and Dickson travel to the 44th Retrovirus meeting at Cold Spring Harbor Laboratories, where they speak with John Coffin, Stephen Hughes, Ya-Chi Ho, and Matt Takata about the meeting and their work on HIV-1. Hosts: Vincent Racaniello and Dickson Despommier Guests: John Coffin, Stephen Hughes, Ya-Chi Ho, and Matt Takata Become a patron of TWiV! Links for this episode Cold Spring Harbor Retrovirus Meeting 2018 Retroviruses and cranberries (TWiV 320) Identical proviruses in HIV-1 reservoir (PLoS Path) Measuring HIV-1 latency (PNAS) CG dinucleotide suppression is host defense (Nature) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The TWiV team considers whether those who can do, can't teach, and newly discovered viruses of planarians and Aplysia with the largest RNA genomes. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler and Brianne Barker Become a patron of TWiV! Links for this episode Support Viruses Gordon Conference KNAW Beijerinck Prize Bioterrorism and Biocrimes by Seth Carus (free pdf) History of Biological Warfare by Seth Carus (free) Those Who Can Do, Can't Teach (NYTimes) Planarian nidovirus (bioRxiv) Aplysia nidovirus (bioRxiv) Image credit Letters readon TWiV 509 Weekly Science Picks Brianne- Climate change visualization Alan- Anova sous vide cooker Dickson- Fire season in western US Kathy- Sunscreen in UV and The Projector Illusion Vincent - The Usefulness of Useless Knowledge Listener Pick River- The Conscience of the Present Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Jens returns to present a brief history of bioweapons, with a focus on the program in the Soviet Union, the largest ever undertaken, and his experience working in the decommissioned Soviet bioweapons laboratory known as Vector. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, and Kathy Spindler Guest: Jens Kuhn Become a patron of TWiV! Links for this episode Russia's Bioweapons Labs (Nature) Medical Aspects of Biological Warfare The Soviet Biological Weapons Program by Leitenberg, Zilinskas, Kuhn Biological Threats in the 21st Century by Lentzos Bioterrorism and Biocrimes by W. Seth Carus Russia's Secret Weapons (Nature) Image credit Weekly Science Picks Jens - Nudibranchs, Beastly Garden, large nidovirus (one, two) Rich - Living inside a canister Dickson - Global rivers and streamsKathy - TWiM 181 Dr. Warhol's Periodic Table of Microbes Vincent - Twitter Bots and Russian Trolls Amplify Vaccine Debate Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv