Bloody Minded Episode 80 - GATTTACA | In The Lab: Introduction to Molecular Haematology
Mar 3, 2026
Clear walkthrough of DNA, RNA and the central dogma. A tour of PCR variants, from endpoint to quantitative and digital droplet PCR. Explanations of fragment analysis, melt curves, Sanger and next generation sequencing. Discussion of variant allele frequency, sample handling and lab governance for molecular haematology.
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Coordinate Sampling For RNA Tests To Avoid Failures
- Know sample requirements and quality before ordering tests: DNA is robust but RNA is fragile, so coordinate extractions and timing especially for RNA-based assays.
- Avoid scheduling marrow for RNA tests on Fridays if receiving lab is remote or closed over weekend.
PCR Fundamentals And Why Taq Polymerase Matters
- PCR amplifies a specific DNA region using forward and reverse primers across cycles of denaturation, annealing, and extension.
- Thermostable polymerases like Taq allow automated thermocycling without adding enzyme each cycle.
Match PCR Technique To Clinical Question
- Choose PCR method to match the question: use endpoint PCR for presence/absence and qPCR for quantification (MRD, viral loads).
- Design primers/probes specifically for known sequences; wrong target gives false negatives.