If we’re going to truly understand myeloid disease, we must dispense with the ward and journey to the dystopian heart that beats at the centre of each dysplastic cell. Learn what is valid and in-valid as we swim across an ocean of concepts - past the basic principles of DNA and RNA, Polymerase Chain Reaction and its many many variants, Fragment Analysis and Next Generation Sequencing. Just save a little something for the swim back to clinic next week.
PS The Extra T is for TYOTT
Timestamps
0:55 - Back in the lab, baby!
2:08 - Today's outline
6:24 - What IS molecular testing?
8:13 - The central dogma of molecular biology
14:46 - Types of genetic mutations
20:05 - Nomenclature for gene variants (advanced)
23:52 - Samples for testing
27:42 - What comes after DNA extraction
30:11 - What is PCR and how did we discover it?
38:45 - What we do post PCR
41:27 - Quantitative and RT-PCR
46:54 - Asking the RIGHT question
48:55 - Digital droplet PCR
50:44 - Electrophoresis
52:35 - Fragment analysis and melt curve analysis
54:33 - NGS
1:00:02 - What is a VAF?
1:05:23 - Lab governance (for haem ATs)
1:09:32 - Take home messages
Support the show
Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au
