The Stem Cell Podcast

Guest: Dr. Prisca Liberali is a Senior Group Leader at the Friedrich Miescher Institute for Biomedical Research and an Assistant Professor at the University of Basel. Her research aims to understand the collective properties of organoid systems and their patterns, including how these systems allow systematic perturbation by established methods for modulating gene expression. She studies self-organization, symmetry breaking, intestinal organoid development, gastruloids, and mechanosensing mechanisms. Featured Products and Resources: CRISPR-Cas9 Genome Editing of Human Intestinal Organoids Add Intestinal Organoids to Your Research with IntestiCult™ The Stem Cell Science Round Up Synthetic Embryo Develops Beating Heart – Researchers have created synthetic mouse embryos that show evidence of a brain and a beating heart. A Step Forward for Non-Human Primate iPSC Research – Scientists have shown the initial viability of an embryo that contains both chimpanzee and macaque cells. A Step Towards Human Kidney Organoids – Researchers have developed a highly efficient, serum-free method for differentiating hPSCs into uretic bud organoids and functional collecting duct cells. A Role for Mitochondrial Fission in the Expansion of Muscle Stem Cells – New research shows that mitochondrial dynamics are essential for the successful regenerative capacity of satellite cells. Photo Reference: Courtesy of Dr. Prisca Liberali Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guests: Drs. Tenneille Ludwig, Peter Andrews, and Madeline Lancaster are members of the ISSCR Standards Initiative for Pluripotent Stem Cell Research. They discuss the need for basic and preclinical standards to ensure rigor and reproducibility in stem cell research. They also talk about standards for pluripotency and model systems, and the initiative’s international focus. Featured Products and Resources: Take Your Cells Further with mTeSR™ Plus. Explore Ways to Assess Your Human Pluripotent Stem Cells The Stem Cell Science Round Up Growing Synthetic Embryos – Scientists generated synthetic mouse embryos from naive ESCs. Live Sequencing to Preserve Cell Viability – Live-seq enables single-cell transcriptome profiling as well as downstream molecular and functional analyses on the same cell at distinct time points. An Atlas for the Failing Heart – Researchers performed single-nucleus RNA sequencing on heart samples from patients with cardiomyopathies and controls without structural heart disease. Pluripotency-Associated MAPs Shared Between iPSCs and Cancer Cells – Scientists identified a set of pluripotency-associated MHC I-associated peptides (MAPs) absent from the transcriptome of normal tissues and adult stem cells, but expressed in PSCs and multiple adult cancers. Photo Reference: Courtesy of Drs. Tenneille Ludwig, Peter Andrews, and Madeline Lancaster Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Jackie Damen is a currently a Principal Scientist in Scientific Support for Hematology at STEMCELL Technologies, and was previously the Director of Contract Assay Services. She talks about the company’s culture and growth, and how in her role, she teaches scientists and helps them conduct their experiments. She also discusses systems for drug screening and discovery, opportunities in industry, and her affinity for microscopy and photography. Featured Products and Resources: Keep current with the latest in cell biology research with STEMCELL Science News. STEMCELL is hiring! Explore more than 100 current openings. The Stem Cell Science Round Up Organoids to Model Polycystic Kidney Disease – Scientists developed homogenous organoids to study autosomal dominant polycystic kidney disease and identify drug candidates. Generating Mature Cardiomyocytes – Researchers used cardiac mimetic matrix to generate cardiomyocytes with adult-like characteristics and model pathological hypertrophy. Tracking T Cells in Cancer Organoids – Scientists live-tracked more than 150,000 engineered T cells in patient-derived solid-tumor organoids and identified behavioral clusters. Mesenchymal Stem Cells in the Aorta-Gonad-Mesonephros – PDGFRA+ cells contribute to the aorta-gonad-mesonephros endothelium and transplantable hematopoietic stem cells. Photo Reference: Courtesy of Dr. Jackie Damen Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Marian Hettiaratchi is an Assistant Professor at the Knight Campus at the University of Oregon. Her research focuses on affinity-based biomaterials to promote tissue and bone repair. She talks about the delivery vehicles for these biomaterials, and her recent work on heparin-mediated delivery of BMP-2 to promote bone regeneration. Featured Products and Resources: Keep current with the latest in cell biology research with STEMCELL Science News. STEMCELL is hiring! Explore more than 100 current openings. The Stem Cell Science Round Up DNA Replication Errors in Preimplantation Embryos – DNA replication stress in human preimplantation embryos predisposes gene-poor regions to fragility and to the formation of aneuploidies, impairing developmental potential. Stem Cell Movement in the Intestine – The number of effective stem cells in small intestinal crypts is determined by Wnt-dependent retrograde cellular movement. Inflammation Affects HSC Self-Renewal – Inflammation and infection irreversibly depletes functional HSCs. Colon Cancer Stem Cell Dormancy – Researchers used a lineage-tracing system to identify LGR5+ cancer stem cells that drive cancer growth after chemotherapy. Photo Reference: Courtesy of Dr. Marian Hettiaratchi Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Lydia Finley is an Assistant Member of the Cell Biology Program at Memorial Sloan Kettering Cancer Center. The Finley lab investigates how cellular metabolic pathways regulate cell fate decisions in stem cells and cancer cells. She talks about the metabolic profiles and needs of different cell types, her lab’s discovery of an alternate TCA cycle, and how track and field led her to pursue metabolism research. Featured Products and Resources: We want your feedback! Fill out our survey for a chance to win a bluetooth speaker. STEMCELL is hiring! Explore more than 100 current openings. The Stem Cell Science Round Up Chemical Induction of Mouse Totipotent Stem Cells – Researchers used three small molecules to induce totipotent stem cells from mouse PSCs. Aneuploidy in T Cell Therapy – Scientists found that aneuploidy and chromosomal truncations are frequent outcomes of CRISPR–Cas9 cleavage. Modeling Nipah and Hendra Virus Infections – Researchers modeled viral infection of the human vasculature in PSC-derived artery and vein cells. A Gatekeeper Transcription Factor for Pancreatic Differentiation – CRISPR screening showed that HHEX suppresses liver differentiation to promote pancreatic lineage commitment. Photo Reference: Courtesy of Dr. Lydia Finley Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

In June 2022, we attended the International Society for Stem Cell Research (ISSCR) annual meeting in San Francisco. We spoke with delegates to get their impressions of the meeting. They discussed the most memorable research presented and shared their thoughts on the challenges facing the stem cell field. Featured Products and Resources: Which organoid are you? Take the quiz! If you need help translating your research to the clinic, the Services for Cell Therapy team at STEMCELL Technologies can support you. Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

In June 2022, we attended the International Society for Stem Cell Research (ISSCR) annual meeting in San Francisco, and recorded daily episodes discussing highlights of the previous 24 hours. Here is the final of four special episodes from the meeting. Featured Products and Resources: Explore on-demand webinars on your favorite topics in stem cell research. Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

In June 2022, we attended the International Society for Stem Cell Research (ISSCR) annual meeting in San Francisco, and recorded daily episodes discussing highlights of the previous 24 hours. Here is the third of four special episodes from the meeting. Featured Products and Resources: The Stem Cell Research Standards Task Force is a half-day meeting on Sunday, June 19 that will explore the developing quality standards for pluripotent stem cell research. Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

In June 2022, we attended the International Society for Stem Cell Research (ISSCR) annual meeting in San Francisco, and recorded daily episodes discussing highlights of the previous 24 hours. Here is the second of four special episodes from the meeting. Featured Products and Resources: Access free, self-paced virtual training courses from STEMCELL Technologies. Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

In June 2022, we attended the International Society for Stem Cell Research (ISSCR) annual meeting in San Francisco, and recorded daily episodes discussing highlights of the previous 24 hours. Here is the first of four special episodes from the meeting. Featured Products and Resources: View STEMCELL’s Virtual Conference Exhibition Subscribe to our newsletter! Never miss updates about new episodes. Subscribe