Bloody Minded Episode 70 - The Wizard of Clots | In the Lab: Thrombophilia Testing I
Nov 25, 2025
Explore the fascinating world of thrombophilia testing with engaging insights from laboratory experts. Discover the nuances of lupus anticoagulant testing and how various assays differentiate between deficiencies. Learn about the biology of Protein C and S, including their functions and the intricacies of testing methods like ELISA and chromogenic assays. The discussion dives into the effects of anticoagulants on test results and debunks common myths around coagulation. Buckle up for a thrilling journey through the emerald lab!
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Prefer Chromogenic Protein C Assays
- Use chromogenic protein C assays that activate protein C with Protac to measure activity when possible.
- Remember clot-based protein C assays exist but are more prone to interference by anticoagulants and LA.
What Chromogenic Protein C Shows
- Chromogenic protein C assays measure activated protein C cleavage of a colorimetric substrate and report activity.
- These assays detect functional protein C but cannot distinguish type 1 (low antigen) from some type 2 defects.
Clot-Based Protein C Pros and Cons
- Clot-based protein C assays activate protein C and then measure clotting times; they can detect some type 2 defects missed by chromogenic tests.
- However, clot-based assays are more susceptible to DOACs, LA and other interferences.